SnaBI from New England Biolabs

Product Specs
ItemSnaBI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0130L
Quantity2500 units
Concentration5-25 u/µL
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

TAC/GTA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) LRP8‐mediated selenocysteine uptake is a targetable vulnerability in MYCN‐amplified neuroblastomaEMBO Molecular MedicineAugust 7, 2023Hamed Alborzinia, Zhiyi Chen, Umut Yildiz, Florencio Porto Freitas, Felix C E Vogel, Julianna Patricia Varga, Jasmin Batani, Christoph Bartenhagen, Werner Schmitz, Gabriele Büchel, et al.sub‐cloned via restriction digest of the plasmid with NsiI‐HF (NEB, Cat. No. R3127) and SnaBI (NEB, Cat. No. R0130) and the insertion of a synthetic dsDNA fragment coding for a gRNA scaffold sequence with MS2 stem‐loop

(2) EtcPRS (Mut) as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondiiiScienceApril 21, 2023Sun P, Zhang Y, Wang C, Hu D, Liu J, Chen L, Shi F, Tang X, Hao Z, Suo J, et al.buffer,NEW ENGLAND Biolabs,B9027S Percoll,CYTIVA,17089101 SnaBI restriction enzyme,NEW ENGLAND Biolabs,R0130L 4% paraformaldehyde (PFA),NOVON,SS0312 RIPA,Macgene,MP015 DMSO,Applichem,A3672 0.25% Triton X-100,Sigma,MC0711 Albumin

(3) Genetic tools for the stable overexpression of circular RNAsRNA BiologyDecember 31, 2022Nicol Mecozzi, Arianna Nenci, Olga Vera, Ilah Bok, Aimee Falzone, Gina M. DeNicola, Florian A. KarrethGeneArt, followed by PCR amplification and InFusion cloning into SnaBI/PstI (New England Biolabs, Cat. #s R0130 and R3140, respectively) digested EF1α-spGFP-ZKSCAN1-Blast transposon plasmid. Cell culture Human

(4) System-Wide Analysis of the GATC-Binding Nucleoid-Associated Protein Gbn and Its Impact on Streptomyces DevelopmentmSystemsJune 28, 2022Chao Du, Joost Willemse, Amanda M. Erkelens, Victor J. Carrion, Remus T. Dame, Gilles P. van Wezelno. R3136), XbaI (R0145), EcoRI-HF (R3101), HindIII-HF (R3104), BbsI (R0539), NcoI-HF (R3193), SnaBI (R0130), StuI (R0187), SacI (R0156), and NdeI (R0111). Phusion polymerase (M0532) and T4 DNA ligase (M0202)

(5) Protocol for design, construction, and selection of genome phage (gPhage) display librariesSTAR ProtocolsDecember 17, 2021Luis Antonio Rodriguez Carnero, AndrÃ© Azevedo Reis Teixeira, Fenny Hui Fen Tang, AndrÃ©ia Kuramoto, Maria JÃºlia Manso Alves, Walter Colli, JoÃ£o Carlos Setubal, EdÃ©cio Cunha-Neto, Renata Pasqualini, Wadih Arap, et al.broth,Difco,Cat#262200 Carbenicillin,Sigma,Cat#C1389-5G Penicillin,Sigma,Cat#D7794-100MU Streptomycin,Sigma,Cat#S9137-100G CsCl,Sigma,Cat#V000561 Ethidium bromide,Thermo Fisher Scientific,Cat#15585-011 Eco105I,New England BioLabs,Cat#R0130L FastAP,Thermo Fisher Scientific,Cat#EF0651 Low-melting point agarose,Sigma,Cat#A9539-500G T4 DNA ligase

(6) Targeted Enrichment of rRNA Gene Tandem Arrays for Ultra-Long Sequencing by Selective Restriction Endonuclease DigestionFrontiers in Plant ScienceApril 28, 2021Anastasia McKinlay, Dalen Fultz, Feng Wang, Craig S. Pikaardthe restriction endonucleases BstZ17I-HF (NEB #R3594), SpeI-HF (NEB #R3133), BclI-HF (NEB #R3160), SnaBI (NEB #R0130), MscI (NEB #R0534), and PvuII-HF (NEB #R3151) at 37◦ C overnight. The buffer was then removed and replaced

(7) SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by AktDevelopmental cellApril 19, 2021Burckhardt CJ, Minna JD, Danuser G.puromycin resistance genes follow after an internal ribosomal entry site. For subcloning SnaBI (NEB, R0130) and NotI-HF (NEB, R3189) were used. The same approach was used for Dyn2-GFP, Eps15-GFP, Eps15-SNAP,

(8) Engineering SARS-CoV-2 using a reverse genetic systemNature ProtocolsMarch 1, 2021Xuping Xie, Kumari G. Lokugamage, Xianwen Zhang, Michelle N. Vu, Antonio E. Muruato, Vineet D. Menachery, Pei-Yong Shino. R0734L) Restriction enzyme PvuI-HF (NEB, cat. no. R3150S) Restriction enzyme SnaBI (NEB, cat. no. R0130L) Ribonucleotide solution mix (rNTP solution mix; NEB, cat. no. N0466L) SOC outgrowth medium (10 mL;

(9) Separable functions of Tof1/Timeless in intra-S-checkpoint signalling, replisome stability and DNA topological stressNucleic Acids ResearchDecember 2, 2020Rose Westhorpe, Andrea Keszthelyi, Nicola E Minchell, David Jones, Jonathan Baxterpurified DNA was digested with BamHI-HF (New England Biolabs, R3136S) and SnaBI (New England Biolabs, R0130L) or SnaBI alone according to manufacturer's instructions. After nicking/digestion the DNA was precipitated,

(10) Tissue-Specific In Vivo Biotin Chromatin Immunoprecipitation with Sequencing in Zebrafish and ChickenSTAR ProtocolsOctober 28, 2020Martyna Lukoseviciute, Irving T.C. Ling, Upeka Senanayake, Ivan Candido-Ferreira, Gunes Taylor, Ruth M. Williams, Tatjana Sauka-SpenglerL4509CAS: 151-21-3 High Fidelity (HF) NcoI,NEB,Cat#R3193S SnaBI Restriction endonuclease,NEB,Cat#R0130 High Fidelity (HF) EcoRV,NEB,Cat#R3195 "Proteinase K, recombinant, PCR Grade",Roche,Cat#3115836001 "RNase A, DNase and protease-free (10 mg/mL

(11) Mechanical regulation of glycolysis via cytoskeleton architectureNatureFebruary 1, 2020Park JS, Burckhardt CJ, Lazcano R, Solis LM, Isogai T, Li L, Chen CS, Gao B, Minna JD, Bachoo R, et al.subcloned from pEGFP vectors into pLVX puro or neo (632183, 832181, Clontech) vectors using SnaBI (R0130, NEB) and NotI (R3189S) restriction sites. For shRNA mediated protein knockdown vectors from the pGIPZ

(12) A Novel Animal Model of Emphysema Induced by Anti-Elastin AutoimmunityJournal of immunology (Baltimore, Md. : 1950)July 15, 2019Gu BH, Sprouse ML, Madison MC, Hong MJ, Yuan X, Tung HY, Landers CT, Song LZ, Corry DB, Bettini M, et al.specific restriction sites digestion: BstBI (NEB #R0519) and MfeI (NEB #R3589) for TCRα, and SnaBI (NEB #R0130) and SacII (NEB #R0157) for TCRβ. Following confirmation of the correct insertion sites, Sanger Sequencing

(13) Rapid identification and expression of human TCRs in retrogenic mice☆Journal of Immunological MethodsDecember 1, 2016Maran L. Sprouse, Gabriele Blahnik, Thomas Lee, Natalie Tully, Pinaki Banerjee, Eddie A. James, Maria J. Redondo, Matthew L. Bettini, Maria Bettiniregion. Purified alpha nested PCR products and beta containing TCR vectors were digested with SnabI (NEB #R0130L) and SacII (NEB #R0157S), and ligated. Stable retroviral producer cell lines were generated as previously

(14) Cell line with endogenous EGFRvIII expression is a suitable model for research and drug development purposesOncotargetMay 31, 2016Stec WJ, Rosiak K, Siejka P, Peciak J, Popeda M, Banaszczyk M, Pawlowska R, Treda C, Hulas-Bigoszewska K, Piaskowski S, et al.fragment was PCR-amplified, digested with HpaI/SnaBI (both New England Biolabs, Cat. no. R0105 and R0130, respectively) and ligated with pLOC-RFP vector (Thermo Scientific, Cat. no. OHS5922) previously digested

(15) Incorporation of a viral DNA-packaging motor channel in lipid bilayers for real-time, single-molecule sensing of chemicals and double-stranded DNANature ProtocolsFebruary 1, 2013Farzin Haque, Jia Geng, Carlo Montemagno, Peixuan Guoeyes and skin. Harmful if swallowed Restriction enzymes (New England BioLabs Inc.): SnaBI (cat. no. R0130S); ScaI (cat. no. R0122T), BstNI (cat. no. R0168S); EcoRI (cat. no. R0101T); SmaI (cat. no. R0141S);

(16) A genome-wide inducible phenotypic screen identifies antisense RNA constructs silencing Escherichia coli essential genesFEMS Microbiology LettersApril 1, 2012Jia Meng, Gregory Kanzaki, Diane Meas, Christopher K. Lam, Heather Crummer, Justina Tain, H. Howard Xu

(17) An efficient method of directly cloning chimpanzee adenovirus as a vaccine vectorNature ProtocolsNovember 1, 2010Dongming Zhou, Xiangyang Zhou, Ang Bian, Hua Li, Heng Chen, Juliana C Small, Yan Li, Wynetta Giles-Davis, Zhiquan Xiang, Hildegund C J Ertlcat. no. R0547S) XbaI (New England Biolabs, cat. no. R0145S) SnaBI (New England Biolabs, cat. no. R0130S) NdeI (New England Biolabs, cat. no. R0111S) SphI (New England Biolabs, cat. no. R0182S) HindIII

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SnaBI

SnaBI from New England Biolabs

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SnaBI from New England Biolabs

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